Surface topography of histidine residues in lysozymes
نویسندگان
چکیده
منابع مشابه
Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography.
Immobilized metal ion affinity chromatography (IMAC) has been explored as a probe into the topography of histidyl residues of a protein molecule. An evaluation of the chromatographic behavior of selected model proteins--thioredoxin, ubiquitin, calmodulin, lysozyme, cytochrome c, and myoglobin on immobilized transition metal ions (Co2+, Ni2+, Cu2+, and Zn2+)--allows establishment of the followin...
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Natural abundance *‘C NMR (at 67.9 MHz) is used to study the nonprotonated aromatic carbons of bovine pancreatic ribonuclease A. The CY resonances of the 4 histidines are identified and assigned to specific residues. The effect of pH on the chemical shifts of these resonances yields information about the tautomeric state of the imidazole form of each histidine residue. Only His-119 exists predo...
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Ticks are blood feeding arthropod parasites transmitting a wide variety of pathogens to their vertebrate hosts. The vector capability of ticks is tightly linked to their immune system. Ticks can defend themselves against microbial infection with a variety of antimicrobial peptides comprising lysozymes, defensins, and unique molecules not found in other arthropods. Lysozymes are hydrolytic enzym...
متن کاملInvolvement of histidine residues in proton sensing of ROMK1 channel.
ROMK channels are inhibited by intracellular acidification. This pH sensitivity is related to several amino acid residues in the channel proteins such as Lys-61, Thr-51, and His-206 (in ROMK2). Unlike all other amino acids, histidine is titratable at pH 6-7 carrying a positive charge below pH 6. To test the hypothesis that certain histidine residues are engaged in CO(2) and pH sensing of ROMK1,...
متن کاملHistidine Hydrogen-Deuterium Exchange Mass Spectrometry for Probing the Microenvironment of Histidine Residues in Dihydrofolate Reductase
BACKGROUND Histidine Hydrogen-Deuterium Exchange Mass Spectrometry (His-HDX-MS) determines the HDX rates at the imidazole C(2)-hydrogen of histidine residues. This method provides not only the HDX rates but also the pK(a) values of histidine imidazole rings. His-HDX-MS was used to probe the microenvironment of histidine residues of E. coli dihydrofolate reductase (DHFR), an enzyme proposed to u...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1991
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1991.tb16478.x